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人前列腺增生细胞BPH-1

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人前列腺增生细胞BPH-1
中文名称人前列腺增生细胞BPH-1
英文名称 BPH1; Benign Prostatic Hyperplasia-1
建系背景 prostate epithelial cells from a 68-year-old man with benig n prostate hyperplasia; cells were immortalized with SV-40 large T-antigen; cells were described in the literature to express cytokeratins 8, 18, and 19 (but not 14) and to metabolize prostatic androgens.
组织来源 前列腺
生长特性 贴壁细胞
细胞形态 上皮样细胞
细胞培养条件 RPMI-1640+20% FBS+1% P/S
培养条件气体 空气,95%;CO2,5%;温度:37℃
推荐冻存条件 30% 基础培养基+60%FBS+10%DMSO
冻存液氮 液氮保存
推荐传代比例 1:2-1:4
推荐换液频率 2-3次/周
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