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| 中文名称 | 人前列腺增生细胞BPH-1 |
|---|---|
| 英文名称 | BPH1; Benign Prostatic Hyperplasia-1 |
| 建系背景 | prostate epithelial cells from a 68-year-old man with benig n prostate hyperplasia; cells were immortalized with SV-40 large T-antigen; cells were described in the literature to express cytokeratins 8, 18, and 19 (but not 14) and to metabolize prostatic androgens. |
| 组织来源 | 前列腺 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮样细胞 |
| 细胞培养条件 | RPMI-1640+20% FBS+1% P/S |
| 培养条件气体 | 空气,95%;CO2,5%;温度:37℃ |
| 推荐冻存条件 | 30% 基础培养基+60%FBS+10%DMSO |
| 冻存液氮 | 液氮保存 |
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
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